Journal: Scientific Reports

Article Title: Each protomer of a dimeric YidC functions as a single membrane insertase

doi: 10.1038/s41598-017-18830-9

Figure Lengend Snippet: Insertion of SciP is affected by mutations in the YidC monomer and YidC dimer. ( A ) The insertion of SciP with a cysteine residue at 218 in the C-tail was expressed in MK6 (lanes 1, 2), coexpressed with YidC-ΔCH2 (lanes 3, 4), with YidC-T362A (lanes 5, 6) or with YidC 0 (lanes 7, 8) were pulse-labelled for 3 min. The even-numbered samples were treated with AMS that shifts the protein by 0.5 kDa when the C-terminal tail was translocated to the periplasm. The total cell protein was immunoprecipitated with his tag antiserum and analyzed by SDS-PAGE and phosphorimaging. ( B ) Membrane insertion of SciP was monitored in cells coexpressing various YidC dimers as described above. Lane 1 is the control encoding a cysteine-less YidC in both protomers (C 0 /C 0 ), lane 2: ΔCH2/C 0 , lane 3: C 0 /ΔCH2, lane 4: ΔCH2/ΔCH2, lane 5: T362A/C 0 , lane 6: C 0 /T362A, lane 7: T362A/T362A, lane 8: empty vector control. Quantitation was carried out as described in Methods.

Article Snippet: The band intensity (without gel background) of the inserted protein was quantified using the phosphorimaging Aida Analyzer (Raytest, Straubenhardt).

Techniques: Residue, Immunoprecipitation, SDS Page, Membrane, Control, Plasmid Preparation, Quantitation Assay

Journal: Scientific Reports

Article Title: Each protomer of a dimeric YidC functions as a single membrane insertase

doi: 10.1038/s41598-017-18830-9

Figure Lengend Snippet: Both protomers of a dimeric YidC are active as a membrane insertase to integrate Pf3-Lep protein. ( A ) Schematic of transmembrane Pf3-Lep before and after proteinase K treatment. ( B ) The insertion of Pf3-Lep was coexpressed in MK6 with YidC-C 0 (lanes 1, 2), YidC-ΔCH2 (lanes 3, 4), YidC-T362A (lanes 5, 6) or the empty plasmid (lanes 7, 8) and pulse-labelled for 1 min. The membrane insertion was assayed by proteinase K digestion when added to the periplasmic side of the cells. When the protein is membrane inserted the protease cleavage generates a slightly shifted fragment. The samples were immunoprecipitated with Lep antiserum and analyzed by SDS-PAGE and phosphorimaging. ( C ) Membrane insertion of Pf3-Lep was monitored in cells coexpressing various YidC dimers as described above. Lane1 is the control encoding a cysteine-less YidC in both protomers (C 0 /C 0 ), lane 2: ΔCH2/C 0 , lane 3: C 0 /ΔCH2, lane 4: ΔCH2/ΔCH2, lane 5: T362A/C 0 , lane 6: C 0 /T362A, lane 7: T362A/T362A. Quantitation of translocation of N-tail of Pf3-Lep was performed as described in Methods.

Article Snippet: The band intensity (without gel background) of the inserted protein was quantified using the phosphorimaging Aida Analyzer (Raytest, Straubenhardt).

Techniques: Membrane, Plasmid Preparation, Immunoprecipitation, SDS Page, Control, Quantitation Assay, Translocation Assay

Journal: Journal of Applied Clinical Medical Physics

Article Title: Estimation of primary radiation output for wide‐beam computed tomography scanner

doi: 10.1002/acm2.12598

Figure Lengend Snippet: The CR pixel value as a function of K air or tube current. CR cassettes (24 cm × 30 cm) in conjunction with a photostimulable phosphor plate were exposed under axial scanning at 80 kVp and rotation time of 0.275 s and were processed with a fixed mode with a latitude of 4 and sensitivity of 5 using the AVE4.0 test menu in the CR system that can avoid any raw data manipulation. It shows a log‐linear relationship between the CR pixel value and K air for CT exposure parameters less than or equal to 30 mA (8.25 mAs).

Article Snippet: A CR cassette (24 cm × 30 cm) in conjunction with a photostimulable phosphor plate (Imaging Plate Cassette Type CC and IP ST‐VI; Fujifilm Co., Ltd., Tokyo, Japan) was also utilized in this study.

Techniques: