phosphor imager Search Results


imaging plates bas-tr 2025  (Raytest GmbH)
90
Raytest GmbH imaging plates bas-tr 2025
Imaging Plates Bas Tr 2025, supplied by Raytest GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phosphor+imager/pm15744252-47-14-21?v=Raytest+GmbH
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imaging plates bas-tr 2025 - by Bioz Stars, 2026-06
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phosphor imaging plates raytest-fuji  (Raytest GmbH)
90
Raytest GmbH phosphor imaging plates raytest-fuji
Phosphor Imaging Plates Raytest Fuji, supplied by Raytest GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phosphor+imager/pm18006612-53-6-9?v=Raytest+GmbH
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phosphor imaging plates raytest-fuji - by Bioz Stars, 2026-06
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phosphorimaging aida analyzer  (Raytest GmbH)
90
Raytest GmbH phosphorimaging aida analyzer
Insertion of SciP is affected by mutations in the YidC monomer and YidC dimer. ( A ) The insertion of SciP with a cysteine residue at 218 in the C-tail was expressed in MK6 (lanes 1, 2), coexpressed with YidC-ΔCH2 (lanes 3, 4), with YidC-T362A (lanes 5, 6) or with YidC 0 (lanes 7, 8) were pulse-labelled for 3 min. The even-numbered samples were treated with AMS that shifts the protein by 0.5 kDa when the C-terminal tail was translocated to the periplasm. The total cell protein was immunoprecipitated with his tag antiserum and analyzed by SDS-PAGE and <t>phosphorimaging.</t> ( B ) Membrane insertion of SciP was monitored in cells coexpressing various YidC dimers as described above. Lane 1 is the control encoding a cysteine-less YidC in both protomers (C 0 /C 0 ), lane 2: ΔCH2/C 0 , lane 3: C 0 /ΔCH2, lane 4: ΔCH2/ΔCH2, lane 5: T362A/C 0 , lane 6: C 0 /T362A, lane 7: T362A/T362A, lane 8: empty vector control. Quantitation was carried out as described in Methods.
Phosphorimaging Aida Analyzer, supplied by Raytest GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phosphor+imager/pmc05766580-228-14-17?v=Raytest+GmbH
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phosphorimaging aida analyzer - by Bioz Stars, 2026-06
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coated photoreactive phosphorous fcr imaging plates  (FUJIFILM)
90
FUJIFILM coated photoreactive phosphorous fcr imaging plates
Insertion of SciP is affected by mutations in the YidC monomer and YidC dimer. ( A ) The insertion of SciP with a cysteine residue at 218 in the C-tail was expressed in MK6 (lanes 1, 2), coexpressed with YidC-ΔCH2 (lanes 3, 4), with YidC-T362A (lanes 5, 6) or with YidC 0 (lanes 7, 8) were pulse-labelled for 3 min. The even-numbered samples were treated with AMS that shifts the protein by 0.5 kDa when the C-terminal tail was translocated to the periplasm. The total cell protein was immunoprecipitated with his tag antiserum and analyzed by SDS-PAGE and <t>phosphorimaging.</t> ( B ) Membrane insertion of SciP was monitored in cells coexpressing various YidC dimers as described above. Lane 1 is the control encoding a cysteine-less YidC in both protomers (C 0 /C 0 ), lane 2: ΔCH2/C 0 , lane 3: C 0 /ΔCH2, lane 4: ΔCH2/ΔCH2, lane 5: T362A/C 0 , lane 6: C 0 /T362A, lane 7: T362A/T362A, lane 8: empty vector control. Quantitation was carried out as described in Methods.
Coated Photoreactive Phosphorous Fcr Imaging Plates, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phosphor+imager/10__1017_slash_s0007114524003052-77-6-11?v=FUJIFILM
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coated photoreactive phosphorous fcr imaging plates - by Bioz Stars, 2026-06
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phosphorimager software  (Raytest GmbH)
90
Raytest GmbH phosphorimager software
Insertion of SciP is affected by mutations in the YidC monomer and YidC dimer. ( A ) The insertion of SciP with a cysteine residue at 218 in the C-tail was expressed in MK6 (lanes 1, 2), coexpressed with YidC-ΔCH2 (lanes 3, 4), with YidC-T362A (lanes 5, 6) or with YidC 0 (lanes 7, 8) were pulse-labelled for 3 min. The even-numbered samples were treated with AMS that shifts the protein by 0.5 kDa when the C-terminal tail was translocated to the periplasm. The total cell protein was immunoprecipitated with his tag antiserum and analyzed by SDS-PAGE and <t>phosphorimaging.</t> ( B ) Membrane insertion of SciP was monitored in cells coexpressing various YidC dimers as described above. Lane 1 is the control encoding a cysteine-less YidC in both protomers (C 0 /C 0 ), lane 2: ΔCH2/C 0 , lane 3: C 0 /ΔCH2, lane 4: ΔCH2/ΔCH2, lane 5: T362A/C 0 , lane 6: C 0 /T362A, lane 7: T362A/T362A, lane 8: empty vector control. Quantitation was carried out as described in Methods.
Phosphorimager Software, supplied by Raytest GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phosphor+imager/10__1046_slash_j__1365___2141__2002__03283__x-51-18-21?v=Raytest+GmbH
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phosphorimager software - by Bioz Stars, 2026-06
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denoptix storage phosphor plates  (Gendex labs)
90
Gendex labs denoptix storage phosphor plates
Insertion of SciP is affected by mutations in the YidC monomer and YidC dimer. ( A ) The insertion of SciP with a cysteine residue at 218 in the C-tail was expressed in MK6 (lanes 1, 2), coexpressed with YidC-ΔCH2 (lanes 3, 4), with YidC-T362A (lanes 5, 6) or with YidC 0 (lanes 7, 8) were pulse-labelled for 3 min. The even-numbered samples were treated with AMS that shifts the protein by 0.5 kDa when the C-terminal tail was translocated to the periplasm. The total cell protein was immunoprecipitated with his tag antiserum and analyzed by SDS-PAGE and <t>phosphorimaging.</t> ( B ) Membrane insertion of SciP was monitored in cells coexpressing various YidC dimers as described above. Lane 1 is the control encoding a cysteine-less YidC in both protomers (C 0 /C 0 ), lane 2: ΔCH2/C 0 , lane 3: C 0 /ΔCH2, lane 4: ΔCH2/ΔCH2, lane 5: T362A/C 0 , lane 6: C 0 /T362A, lane 7: T362A/T362A, lane 8: empty vector control. Quantitation was carried out as described in Methods.
Denoptix Storage Phosphor Plates, supplied by Gendex labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phosphor+imager/pmc03068829-3-21-26?v=Gendex+labs
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denoptix storage phosphor plates - by Bioz Stars, 2026-06
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fujifilm-9000 phosphor-image scanner  (FUJIFILM)
90
FUJIFILM fujifilm-9000 phosphor-image scanner
Insertion of SciP is affected by mutations in the YidC monomer and YidC dimer. ( A ) The insertion of SciP with a cysteine residue at 218 in the C-tail was expressed in MK6 (lanes 1, 2), coexpressed with YidC-ΔCH2 (lanes 3, 4), with YidC-T362A (lanes 5, 6) or with YidC 0 (lanes 7, 8) were pulse-labelled for 3 min. The even-numbered samples were treated with AMS that shifts the protein by 0.5 kDa when the C-terminal tail was translocated to the periplasm. The total cell protein was immunoprecipitated with his tag antiserum and analyzed by SDS-PAGE and <t>phosphorimaging.</t> ( B ) Membrane insertion of SciP was monitored in cells coexpressing various YidC dimers as described above. Lane 1 is the control encoding a cysteine-less YidC in both protomers (C 0 /C 0 ), lane 2: ΔCH2/C 0 , lane 3: C 0 /ΔCH2, lane 4: ΔCH2/ΔCH2, lane 5: T362A/C 0 , lane 6: C 0 /T362A, lane 7: T362A/T362A, lane 8: empty vector control. Quantitation was carried out as described in Methods.
Fujifilm 9000 Phosphor Image Scanner, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phosphor+imager/pm29924849-196-1-1?v=FUJIFILM
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fujifilm-9000 phosphor-image scanner - by Bioz Stars, 2026-06
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phosphor image analysis  (FUJIFILM)
90
FUJIFILM phosphor image analysis
Insertion of SciP is affected by mutations in the YidC monomer and YidC dimer. ( A ) The insertion of SciP with a cysteine residue at 218 in the C-tail was expressed in MK6 (lanes 1, 2), coexpressed with YidC-ΔCH2 (lanes 3, 4), with YidC-T362A (lanes 5, 6) or with YidC 0 (lanes 7, 8) were pulse-labelled for 3 min. The even-numbered samples were treated with AMS that shifts the protein by 0.5 kDa when the C-terminal tail was translocated to the periplasm. The total cell protein was immunoprecipitated with his tag antiserum and analyzed by SDS-PAGE and <t>phosphorimaging.</t> ( B ) Membrane insertion of SciP was monitored in cells coexpressing various YidC dimers as described above. Lane 1 is the control encoding a cysteine-less YidC in both protomers (C 0 /C 0 ), lane 2: ΔCH2/C 0 , lane 3: C 0 /ΔCH2, lane 4: ΔCH2/ΔCH2, lane 5: T362A/C 0 , lane 6: C 0 /T362A, lane 7: T362A/T362A, lane 8: empty vector control. Quantitation was carried out as described in Methods.
Phosphor Image Analysis, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phosphor+imager/pmc01808434-162-10-13?v=FUJIFILM
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phosphor image analysis - by Bioz Stars, 2026-06
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phosphor imaging plates bas-iii  (FUJIFILM)
90
FUJIFILM phosphor imaging plates bas-iii
Insertion of SciP is affected by mutations in the YidC monomer and YidC dimer. ( A ) The insertion of SciP with a cysteine residue at 218 in the C-tail was expressed in MK6 (lanes 1, 2), coexpressed with YidC-ΔCH2 (lanes 3, 4), with YidC-T362A (lanes 5, 6) or with YidC 0 (lanes 7, 8) were pulse-labelled for 3 min. The even-numbered samples were treated with AMS that shifts the protein by 0.5 kDa when the C-terminal tail was translocated to the periplasm. The total cell protein was immunoprecipitated with his tag antiserum and analyzed by SDS-PAGE and <t>phosphorimaging.</t> ( B ) Membrane insertion of SciP was monitored in cells coexpressing various YidC dimers as described above. Lane 1 is the control encoding a cysteine-less YidC in both protomers (C 0 /C 0 ), lane 2: ΔCH2/C 0 , lane 3: C 0 /ΔCH2, lane 4: ΔCH2/ΔCH2, lane 5: T362A/C 0 , lane 6: C 0 /T362A, lane 7: T362A/T362A, lane 8: empty vector control. Quantitation was carried out as described in Methods.
Phosphor Imaging Plates Bas Iii, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phosphor+imager/pm31627245-138-10-14?v=FUJIFILM
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phosphor imaging plates bas-iii - by Bioz Stars, 2026-06
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photo-reactive phosphorous imaging plates computed radiography (fcr  (FUJIFILM)
90
FUJIFILM photo-reactive phosphorous imaging plates computed radiography (fcr
Insertion of SciP is affected by mutations in the YidC monomer and YidC dimer. ( A ) The insertion of SciP with a cysteine residue at 218 in the C-tail was expressed in MK6 (lanes 1, 2), coexpressed with YidC-ΔCH2 (lanes 3, 4), with YidC-T362A (lanes 5, 6) or with YidC 0 (lanes 7, 8) were pulse-labelled for 3 min. The even-numbered samples were treated with AMS that shifts the protein by 0.5 kDa when the C-terminal tail was translocated to the periplasm. The total cell protein was immunoprecipitated with his tag antiserum and analyzed by SDS-PAGE and <t>phosphorimaging.</t> ( B ) Membrane insertion of SciP was monitored in cells coexpressing various YidC dimers as described above. Lane 1 is the control encoding a cysteine-less YidC in both protomers (C 0 /C 0 ), lane 2: ΔCH2/C 0 , lane 3: C 0 /ΔCH2, lane 4: ΔCH2/ΔCH2, lane 5: T362A/C 0 , lane 6: C 0 /T362A, lane 7: T362A/T362A, lane 8: empty vector control. Quantitation was carried out as described in Methods.
Photo Reactive Phosphorous Imaging Plates Computed Radiography (Fcr, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phosphor+imager/10__1016_slash_j__aquaculture__2024__741140-88-5-9?v=FUJIFILM
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photo-reactive phosphorous imaging plates computed radiography (fcr - by Bioz Stars, 2026-06
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silver-activated phosphor glass dosimeter  (CT Imaging GmbH)
90
CT Imaging GmbH silver-activated phosphor glass dosimeter
Insertion of SciP is affected by mutations in the YidC monomer and YidC dimer. ( A ) The insertion of SciP with a cysteine residue at 218 in the C-tail was expressed in MK6 (lanes 1, 2), coexpressed with YidC-ΔCH2 (lanes 3, 4), with YidC-T362A (lanes 5, 6) or with YidC 0 (lanes 7, 8) were pulse-labelled for 3 min. The even-numbered samples were treated with AMS that shifts the protein by 0.5 kDa when the C-terminal tail was translocated to the periplasm. The total cell protein was immunoprecipitated with his tag antiserum and analyzed by SDS-PAGE and <t>phosphorimaging.</t> ( B ) Membrane insertion of SciP was monitored in cells coexpressing various YidC dimers as described above. Lane 1 is the control encoding a cysteine-less YidC in both protomers (C 0 /C 0 ), lane 2: ΔCH2/C 0 , lane 3: C 0 /ΔCH2, lane 4: ΔCH2/ΔCH2, lane 5: T362A/C 0 , lane 6: C 0 /T362A, lane 7: T362A/T362A, lane 8: empty vector control. Quantitation was carried out as described in Methods.
Silver Activated Phosphor Glass Dosimeter, supplied by CT Imaging GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phosphor+imager/pmc06333138-247-11-20?v=CT+Imaging+GmbH
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silver-activated phosphor glass dosimeter - by Bioz Stars, 2026-06
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phosphor imager  (Raytest GmbH)
90
Raytest GmbH phosphor imager
Insertion of SciP is affected by mutations in the YidC monomer and YidC dimer. ( A ) The insertion of SciP with a cysteine residue at 218 in the C-tail was expressed in MK6 (lanes 1, 2), coexpressed with YidC-ΔCH2 (lanes 3, 4), with YidC-T362A (lanes 5, 6) or with YidC 0 (lanes 7, 8) were pulse-labelled for 3 min. The even-numbered samples were treated with AMS that shifts the protein by 0.5 kDa when the C-terminal tail was translocated to the periplasm. The total cell protein was immunoprecipitated with his tag antiserum and analyzed by SDS-PAGE and <t>phosphorimaging.</t> ( B ) Membrane insertion of SciP was monitored in cells coexpressing various YidC dimers as described above. Lane 1 is the control encoding a cysteine-less YidC in both protomers (C 0 /C 0 ), lane 2: ΔCH2/C 0 , lane 3: C 0 /ΔCH2, lane 4: ΔCH2/ΔCH2, lane 5: T362A/C 0 , lane 6: C 0 /T362A, lane 7: T362A/T362A, lane 8: empty vector control. Quantitation was carried out as described in Methods.
Phosphor Imager, supplied by Raytest GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phosphor+imager/pm11884455-71-27-29?v=Raytest+GmbH
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Image Search Results


Insertion of SciP is affected by mutations in the YidC monomer and YidC dimer. ( A ) The insertion of SciP with a cysteine residue at 218 in the C-tail was expressed in MK6 (lanes 1, 2), coexpressed with YidC-ΔCH2 (lanes 3, 4), with YidC-T362A (lanes 5, 6) or with YidC 0 (lanes 7, 8) were pulse-labelled for 3 min. The even-numbered samples were treated with AMS that shifts the protein by 0.5 kDa when the C-terminal tail was translocated to the periplasm. The total cell protein was immunoprecipitated with his tag antiserum and analyzed by SDS-PAGE and phosphorimaging. ( B ) Membrane insertion of SciP was monitored in cells coexpressing various YidC dimers as described above. Lane 1 is the control encoding a cysteine-less YidC in both protomers (C 0 /C 0 ), lane 2: ΔCH2/C 0 , lane 3: C 0 /ΔCH2, lane 4: ΔCH2/ΔCH2, lane 5: T362A/C 0 , lane 6: C 0 /T362A, lane 7: T362A/T362A, lane 8: empty vector control. Quantitation was carried out as described in Methods.

Journal: Scientific Reports

Article Title: Each protomer of a dimeric YidC functions as a single membrane insertase

doi: 10.1038/s41598-017-18830-9

Figure Lengend Snippet: Insertion of SciP is affected by mutations in the YidC monomer and YidC dimer. ( A ) The insertion of SciP with a cysteine residue at 218 in the C-tail was expressed in MK6 (lanes 1, 2), coexpressed with YidC-ΔCH2 (lanes 3, 4), with YidC-T362A (lanes 5, 6) or with YidC 0 (lanes 7, 8) were pulse-labelled for 3 min. The even-numbered samples were treated with AMS that shifts the protein by 0.5 kDa when the C-terminal tail was translocated to the periplasm. The total cell protein was immunoprecipitated with his tag antiserum and analyzed by SDS-PAGE and phosphorimaging. ( B ) Membrane insertion of SciP was monitored in cells coexpressing various YidC dimers as described above. Lane 1 is the control encoding a cysteine-less YidC in both protomers (C 0 /C 0 ), lane 2: ΔCH2/C 0 , lane 3: C 0 /ΔCH2, lane 4: ΔCH2/ΔCH2, lane 5: T362A/C 0 , lane 6: C 0 /T362A, lane 7: T362A/T362A, lane 8: empty vector control. Quantitation was carried out as described in Methods.

Article Snippet: The band intensity (without gel background) of the inserted protein was quantified using the phosphorimaging Aida Analyzer (Raytest, Straubenhardt).

Techniques: Residue, Immunoprecipitation, SDS Page, Membrane, Control, Plasmid Preparation, Quantitation Assay

Both protomers of a dimeric YidC are active as a membrane insertase to integrate Pf3-Lep protein. ( A ) Schematic of transmembrane Pf3-Lep before and after proteinase K treatment. ( B ) The insertion of Pf3-Lep was coexpressed in MK6 with YidC-C 0 (lanes 1, 2), YidC-ΔCH2 (lanes 3, 4), YidC-T362A (lanes 5, 6) or the empty plasmid (lanes 7, 8) and pulse-labelled for 1 min. The membrane insertion was assayed by proteinase K digestion when added to the periplasmic side of the cells. When the protein is membrane inserted the protease cleavage generates a slightly shifted fragment. The samples were immunoprecipitated with Lep antiserum and analyzed by SDS-PAGE and phosphorimaging. ( C ) Membrane insertion of Pf3-Lep was monitored in cells coexpressing various YidC dimers as described above. Lane1 is the control encoding a cysteine-less YidC in both protomers (C 0 /C 0 ), lane 2: ΔCH2/C 0 , lane 3: C 0 /ΔCH2, lane 4: ΔCH2/ΔCH2, lane 5: T362A/C 0 , lane 6: C 0 /T362A, lane 7: T362A/T362A. Quantitation of translocation of N-tail of Pf3-Lep was performed as described in Methods.

Journal: Scientific Reports

Article Title: Each protomer of a dimeric YidC functions as a single membrane insertase

doi: 10.1038/s41598-017-18830-9

Figure Lengend Snippet: Both protomers of a dimeric YidC are active as a membrane insertase to integrate Pf3-Lep protein. ( A ) Schematic of transmembrane Pf3-Lep before and after proteinase K treatment. ( B ) The insertion of Pf3-Lep was coexpressed in MK6 with YidC-C 0 (lanes 1, 2), YidC-ΔCH2 (lanes 3, 4), YidC-T362A (lanes 5, 6) or the empty plasmid (lanes 7, 8) and pulse-labelled for 1 min. The membrane insertion was assayed by proteinase K digestion when added to the periplasmic side of the cells. When the protein is membrane inserted the protease cleavage generates a slightly shifted fragment. The samples were immunoprecipitated with Lep antiserum and analyzed by SDS-PAGE and phosphorimaging. ( C ) Membrane insertion of Pf3-Lep was monitored in cells coexpressing various YidC dimers as described above. Lane1 is the control encoding a cysteine-less YidC in both protomers (C 0 /C 0 ), lane 2: ΔCH2/C 0 , lane 3: C 0 /ΔCH2, lane 4: ΔCH2/ΔCH2, lane 5: T362A/C 0 , lane 6: C 0 /T362A, lane 7: T362A/T362A. Quantitation of translocation of N-tail of Pf3-Lep was performed as described in Methods.

Article Snippet: The band intensity (without gel background) of the inserted protein was quantified using the phosphorimaging Aida Analyzer (Raytest, Straubenhardt).

Techniques: Membrane, Plasmid Preparation, Immunoprecipitation, SDS Page, Control, Quantitation Assay, Translocation Assay